The Use of DNA by the Criminal Justice System  January 29, 2021 
and the Federal Role: Background, Current 
Emily J. Hanson 
Law, and Grants 
Analyst in Social Policy 
  
Deoxyribonucleic acid, or DNA, is the fundamental building block for an individual’s entire 
genetic makeup. DNA is a powerful tool for law enforcement inv estigations because each 
 
person’s DNA is different from that of every other individual (except for identical twins). DNA 
can be extracted from many sources, such as hair, bone, teeth, saliva, and blood. DNA samples can be collected at crime 
scenes, from people who might have been present when the crime occurred, and from crime victims. The information 
obtained from these samples is then compared with other DNA profiles to both eliminate and identify suspects in a criminal 
investigation. 
In the 1980s, states began enacting laws that required the collection of DNA samples from offenders convicted of certain 
sexual and other violent crimes. The samples are analyzed and their profiles are entered into state databases. In the late 1980s, 
the Federal Bureau of Investigation (FBI) Laboratory convened a working group of federal, state, and local forensic scientists 
to establish guidelines for the use of forensic DNA analysis in laboratories. The group proposed guidelines that are the basis 
of current national quality assurance standards, and it urged the creation of a national DNA database. In 1994, federal law (34 
U.S.C §12592(a)) authorized the FBI to operate and maintain a national DNA database for DNA profiles collected from 
people under applicable legal authority and samples collected at crime scenes. In 1998, the FBI launched the National DNA 
Index System (NDIS). 
Statutory provisions authorize the collection of DNA samples from certain arrestees and convicted federal offenders, District 
of Columbia offenders, and military convicted offenders. State laws dictate which convicted offenders, and in some states 
arrestees, will have profiles entered into state DNA databases, while federal law dictates the scope of the national database. 
Increasing awareness of the power of DNA to solve crimes and the mandatory collection of DNA from arrestees and 
convicted offenders has resulted in increased demand for DNA analysis, which has resulted in a backlog of casework. In 
addition to solving crimes, DNA analysis can also help exonerate people accused or convicted of crimes they did not commit. 
Congress has authorized several grant programs to provide assistance to state and local governments for forensic sciences. 
Many of the programs focus on providing state and local governments with funding to reduce the backlog of forensic and 
convicted offender DNA samples waiting to be processed and entered into the national database. Other grant programs 
provide funding for related purposes, such as offsetting the cost of providing post-conviction DNA testing. 
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Contents 
Introduction ................................................................................................................... 1 
Background.................................................................................................................... 1 
The National DNA Index System (NDIS) and the Combined DNA Index System 
(CODIS) ................................................................................................................ 2 
DNA Profiles ............................................................................................................ 4 
Rapid DNA Analysis .................................................................................................. 6 
DNA Backlog ........................................................................................................... 7 
Forensic Casework ............................................................................................... 7 
Convicted Offender and Arrestee Samples................................................................ 8 
Sexual Assault Kits (SAKs) ................................................................................... 9 
Federal Law ................................................................................................................... 9 
Quality Assurance and Proficiency Testing Standards ...................................................... 9 
Inclusion and Expungement of DNA Profiles in the NDIS ............................................. 10 
Collection of DNA Samples from Certain Federal, District of Columbia, and Military 
Offenders............................................................................................................. 11 
Post-conviction DNA Testing .................................................................................... 13 
Preservation of Biological Evidence ........................................................................... 16 
Grants for DNA-Related Programs .................................................................................. 16 
Debbie Smith DNA Backlog Grant Program ................................................................ 17 
Sexual Assault Forensic Exam Program ...................................................................... 19 
DNA Training and Education for Law Enforcement, Correctional Personnel, and 
Court Officers Program .......................................................................................... 19 
Kirk Bloodsworth Post-Conviction DNA Testing Grant Program .................................... 19 
DNA Research and Development Grants ..................................................................... 19 
Sexual Assault Kit Initiative (SAKI) ........................................................................... 19 
Emmett Til  Cold Case Investigations Program ............................................................ 20 
Going Forward ............................................................................................................. 21 
 
Tables 
Table 1. Appropriations for DNA-Related Programs, FY2016-FY2021.................................. 20 
 
Contacts 
Author Information ....................................................................................................... 22 
 
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DNA Testing in Criminal Justice: Background, Current Law, and Grants  
 
Introduction 
Deoxyribonucleic acid, or DNA, is the fundamental building  block for an individual’s entire 
genetic makeup. DNA is a powerful tool for law enforcement investigations because each 
person’s DNA is different from that of every other individual (except for identical twins). By 
analyzing selected DNA sequences (cal ed loci), a crime laboratory can develop a profile to be 
used in identifying a suspect. 
DNA can be extracted from many sources, such as hair, bone, teeth, saliva, and blood. Because 
there is DNA in most cel s in the human body, even a minuscule amount of bodily fluid or tissue 
can yield useful information. Obtaining a DNA sample is not complicated; it can be as simple as a 
swab of the inside of the mouth to obtain cheek cel s and white blood cel s in saliva. 
State and federal DNA databases have proved instrumental in solving crimes, reducing the risk of 
wrongful convictions, and establishing the innocence of those who were wrongly convicted. DNA 
evidence is used to solve crimes in two ways: 
  If a suspect is known, a sample of that person’s DNA can be compared to 
biological evidence found at a crime scene. The results of this comparison may 
then help establish whether the suspect was at the crime scene or whether he or 
she committed the crime. 
  If a suspect is not known, biological evidence from the crime scene can be 
analyzed and compared to offender profiles in existing DNA databases to assist 
in identifying  a suspect. Through the use of DNA databases, biological evidence 
found at one crime scene can also be connected to other crime scenes, linking 
them to the same perpetrator or perpetrators. 
This report provides an overview of how DNA is used to investigate crimes and exonerate 
innocent people of crimes they did not commit.1 It also reviews current law related to collecting 
DNA samples, sharing DNA profiles generated from those samples, and providing access to post-
conviction DNA testing. The report also includes a summary of grant programs authorized by 
Congress to assist state and local governments with reducing DNA backlogs, providing post-
conviction DNA testing, and promoting new technology in the field.  
Background 
Federal law authorizes the Federal Bureau of Investigation (FBI) to operate and maintain a 
national database of DNA profiles collected from people under applicable legal authority and 
samples of biological evidence collected at crime scenes. Statutory provisions authorize the 
federal government to collect DNA samples and enter profiles from certain convicted federal 
offenders and arrestees, District of Columbia offenders, and military offenders. State law dictates 
which arrestees and convicted offenders wil  have profiles entered into state DNA databases, but 
federal law dictates which profiles entered into state databases can be uploaded into the national 
DNA database. This means that there may be arrestees and convicted offenders whose DNA 
qualifies for inclusion in a state database but wil  not qualify for inclusion in the national 
database. The intended purpose of the national DNA  database is to compare offender profiles to 
                                              
1 T his report does not include a discussion  of the use of DNA to identify missing persons and unidentified human 
remains, nor does it include an overview of grant programs to state and local governments for developing DNA profiles 
from samples from missing persons, close relatives of missing  persons, or unidentified human remains. For more on 
this issue,  see CRS  Report RL34616, Missing Adults: Background, Federal Program s, and Issues for Congress. 
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crime scene samples in a central repository of DNA profiles to generate leads in criminal 
investigations. 
Increased awareness of the power of DNA testing to solve crimes and the mandatory collection of 
samples from arrestees and convicted offenders has led to increased demand for DNA analysis 
and casework backlogs. In addition to solving crimes, DNA analysis can also help exonerate 
people convicted of crimes they did not commit.  
A Brief Primer on DNA 
DNA is contained within the nucleus of most cel s in the human body.2 A cel ’s  nucleus contains 23 pairs of 
chromosomes  (46 total) and each chromosome  contains long strands of DNA.3  Genes are specific segments of 
these DNA strands.4 Different genes are comprised  of different lengths of DNA. A gene’s locus is the location of 
the particular section of DNA that makes  up a given gene. Genes,  or groups of genes, provide the code for 
inherited traits such as eye color.  There are different variations of any given gene (e.g., genes for blue eyes  or 
brown eyes), and these variants are cal ed al eles.  The ful  set of DNA for any given person is their genome. 
Genes can be broken down into coding and non-coding genes.5 Coding genes make  up a little  more than 1% of the 
human genome,  and these contain DNA which codes for proteins that are eventual y assembled  into cel s,  tissues, 
and organs.6 Non-coding genes comprise  the remainder  of the human genome and handle vital functions such as 
regulating gene activity.7 Currently, the FBI col ects 20 loci for non-coding genes; each locus contains one or two 
al eles,  and thus an FBI DNA profile could include up to 40 al eles.8    
The National DNA Index System (NDIS) and the Combined DNA 
Index System (CODIS) 
In the 1980s, states began enacting laws that required the collection of DNA samples from 
offenders convicted of certain sexual offenses and other violent crimes. The samples were then 
analyzed and their profiles entered into state databases to identify suspects in criminal 
investigations. In the late 1980s, the FBI Laboratory convened a working group of federal, state, 
and local forensic scientists to establish guidelines for the use of forensic DNA analysis in 
laboratories. The working group proposed guidelines that are the basis for the current national 
Quality Assurance Standards (QAS) and urged the creation of a national DNA database.9 In 1994, 
Congress authorized the FBI to establish and oversee the National DNA Index System (NDIS).10 
                                              
2 U.S.  Department of Health and Human Services, U.S.  National Library of Medicine, “What is DNA?” 
https://medlineplus.gov/genetics/understanding/basics/dna/#:~:text=
DNA%20bases%20pair%20up%20with,spiral%20called%20a%20double%20helix .  
3 U.S.  Department of Health and Human Services, U.S.  National Library of Medicine, “ How Many Chromosomes do 
People Have?”, https://medlineplus.gov/genetics/understanding/basics/howmanychromosomes/. 
4 U.S.  Department of Health and Human Services, U.S.  National Library of Medicine, “ What is a Gene?”, 
https://medlineplus.gov/genetics/understanding/basics/gene/. 
5 U.S.  Department of Health and Human Services, U.S.  National Library of Medicine, “ What is Noncoding DNA?”, 
https://medlineplus.gov/genetics/understanding/basics/noncodingdna/  (hereinafter, “ What is Noncoding DNA?”). 
6 What is Noncoding DNA?  
7 What is Noncoding DNA?  
8 Jules  Epstein, “Genetic Surveillance—T he Bogeyman Response to Familial DNA Investigations,” University of 
Illinois Journal of Law, Technology and Policy, vol. 2009, no. 1, (2009), p. 143. 
9 Statement of Dwight E. Adams, Deputy Assistant Director, Laboratory Division, Federal Bureau  of Investigation, in 
U.S.  Congress, House  of Representatives, Government Reform Committee, Subcommittee on Government Efficiency, 
Financial Management and Intergovernmental Relations, How Effective are State and Federal Agencies Working 
Together to Im plem ent the Use of New  DNA Technologies?, hearing, 107th Cong., 1st sess., March 29, 2004, pp. 53-54. 
10 Index to Facilitate Law Enforcement Exchange of DNA Identification Information, P.L. 103-322, T itle XXI (C).  
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When the NDIS launched in 1998, nine states participated.11 Currently, laboratories in al  50 
states, the District of Columbia, the DC/FBI, Puerto Rico, and the U.S. Army Criminal 
Investigation Laboratory participate in the NDIS.12 
DNA profiles generated by laboratories operated by local law enforcement agencies are stored in 
Local DNA Index Systems (LDISs). DNA profiles generated by state laboratories, along with 
authorized profiles stored in participating LDISs, are uploaded into State DNA Index Systems 
(SDISs). Each state has its own laws specifying which profiles can be included in their SDIS.13 
DNA profiles generated by federal laboratories, along with authorized DNA profiles in 
participating SDISs, are uploaded into the NDIS.14 Federal law dictates which DNA profiles can 
be stored in the NDIS (see below). The NDIS al ows participating laboratories to compare DNA 
on the national level while the SDISs al ow each state to compare DNA profiles stored at the state 
level. Laboratories upload qualifying DNA profiles into an LDIS, an SDIS, or the NDIS and then 
compare DNA profiles using the Combined DNA Index System (CODIS) software produced and 
distributed by the FBI.15 
CODIS can conduct searches to generate investigative leads in the NDIS across three indexes: 
convicted offenders, arrestee, and forensic. The convicted offender index contains DNA profiles 
developed from samples collected from convicted offenders; the arrestee index contains DNA 
profiles developed from samples collected as a result of an arrest; and the forensic index contains 
DNA profiles developed from samples of biological evidence (e.g., blood, semen, or saliva) 
collected at crime scenes or from crime victims.16 As of October 2020, more than 200 labs 
participated in the NDIS nationwide and the database included more than 14 mil ion  offender 
profiles, more than 4 mil ion  arrestee profiles, and more than 1 mil ion  forensic profiles.17 CODIS 
searches across these indexes for potential matches (also referred to as “hits”).18 Matches can 
occur between either the convicted offender or arrestee indexes and the forensic index, thereby 
providing law enforcement with the identity of one or more suspects.19 Also, matches can occur 
between DNA profiles in the forensic index, thereby linking crime scenes to each other and 
identifying serial offenders.20 Matches between multiple samples in the forensic index can al ow 
                                              
11 John M. Butler, Fundamentals of Forensic DNA Typing (Burlington, MA: Academic  Press, 2010), p. 265 
(hereinafter, “ Fundamentals of Forensic DNA T yping”). 
12 Information on the number of participant laboratories and the number of profiles they have uploaded  into the NDIS 
can be  found on the FBI’s website  at https://www.fbi.gov/services/laboratory/biometric-analysis/codis/ndis-statistics. 
13 T he National Conference of State Legislatures (NCSL)  maintains a searchable database  of state DNA laws,  including 
laws  related to which convicted offenders are required  to submit a sample for inclusion in the state’s DNA database and 
whether, and if so, from whom, collects DNA samples from individuals  arrested for certain crimes. T he NCSL’s 
database  is available online at http://www.ncsl.org/research/civil-and-criminal-justice/dna-laws-database.aspx.   
14 U.S.  Department of Justice, Federal Bureau  of Investigation, CODIS—NDIS Statistics, https://www.fbi.gov/services/
laboratory/biometric-analysis/codis/ndis-statistics, (hereinafter, “CODIS-NDIS Statistics”). 
15 U.S.  Department of Justice, Federal Bureau  of Investigation, Frequently Asked Questions on CODIs  and NDIS, 
https://www.fbi.gov/services/laboratory/biometric-analysis/codis/codis-and-ndis-fact -sheet , (hereinafter, “ CODIS 
FAQs”). 
16 National Institute of Justice, DNA Evidence Basics: Types of Samples Suitable for DNA  Testing , https://nij.ojp.gov/
topics/articles/dna-evidence-basics-types-samples-suitable-dna-testing#:~:text=
Questioned%20or%20unknown%20samples%20collected,vaginal%2Fcervical%20sample s%20collected%20on.  
17 CODIS-NDIS  Statistics 
18 CODIS  FAQs. 
19 CODIS  FAQs.  If an offender hit is obtained, that information typically is used as  probable cause  to obtain a new 
DNA sample from that suspect so the match can be confirmed by the crime laboratory  before an arrest is made.  
20 CODIS  FAQs. 
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law enforcement agencies in different jurisdictions to coordinate their efforts and share leads. No 
names or other personal identifiers for offender and arrestee DNA profiles are stored in the NDIS, 
so when a match is made in CODIS, the laboratories that submitted the DNA profiles to the NDIS 
are notified of the match and they contact each other to verify the match and coordinate their 
efforts.21 
DNA Profiles 
DNA profiles entered into CODIS are based on 20 core short tandem repeat (STR) loci selected 
by the FBI’s CODIS Core Loci Working Group.22 In 2017, the number of required loci was 
increased from 13 to 20 to enhance discriminatory power, facilitate international cooperation via 
the collection of more common loci, and aid in missing person investigations.23 The 20 STR loci 
used by the FBI are non-coding, meaning that they are not associated with human attributes such 
as height, eye or skin color, or susceptibility to a particular disease.24 Each locus has one or two 
al eles, and it is these 20 pairs of al eles that are compared to match samples in the forensic index 
with profiles in either the offender or arrestee indexes. The 20 core loci chosen by the FBI 
provide a high level of discriminatory power. Two random Americans wil , on average, share two 
or three al eles.25 The probability that two unrelated individuals wil  share the original 13 pairs of 
al eles  is estimated to be one in several hundred bil ion.26 The new standard of 20 loci is expected 
to improve discriminatory power by eight orders of magnitude.27 
It is important to ensure the quality of the DNA profiles entered into the NDIS. The FBI helps 
ensure the quality of DNA profiles included in the NDIS by signing memorandums of 
understanding with state laboratories whereby the laboratory agrees to adhere to the FBI’s QAS 
(see “Quality Assurance and Proficiency Testing Standards”).28 Laboratories submitting DNA 
profiles to the NDIS must be accredited and audited annual y.29 Annual audits can be conducted 
by either an internal or external auditor, but laboratories must be audited by an external agency at 
least once every two years.30 Laboratories that do not pass the annual audit can be prevented from 
entering DNA profiles in CODIS.31 Currently, the following groups are approved to conduct 
audits: the American Association for Laboratory Accreditation (A2LA) and the ANSI-ASQ 
                                              
21 CODIS  FAQs.  Beyond the DNA profile, the NDIS  does include  an agency identifier, specimen identification 
number, and the DNA laboratory personnel associated with a DNA profile analysis.   
22 U.S.  Department of Justice, Federal Bureau  of Investigation, Combined DNA Index System , https://www.fbi.gov/
services/laboratory/biometric-analysis/codis, (hereinafter “ Combined DNA Index System”). 
23 Combined DNA  Index System. 
24 Jules  Epstein, “Genetic Surveillance—T he Bogeyman Response to Familial DNA Investigations,” University of 
Illinois Journal of Law, Technology and Policy, vol. 2009, no. 1, (2009), p. 143. 
25 Henry T . Greely et al., “Family T ies: T he Use of DNA Offender Databases to Catch Offenders’ Kin,”  Journal of 
Law, Medicine and Ethics, vol. 34, no. 2 (Summer  2006), p. 250 (hereinafter, “ Greely et al., ‘Family T ies’”).  
26 Greely et al., ‘Family T ies’. 
27 Becky Hill  and Margaret Kline, “T he Impact and Benefit of Expanding the U.S. Core Autosomal ST R Markers,” 
presentation at the American Academy of Forensic Sciences,  Washington, DC, February  2013, https://strbase.nist.gov/
pub_pres/AAFS2013-Hill-expanding-core.pdf. 
28 CODIS  FAQs. 
29 U.S.  Department of Justice, Federal Bureau  of Investigation, Quality Assurance Standards for DNA Databasing 
Laboratories, Standards  15 and 17, https://www.fbi.gov/file-repository/quality-assurance-standards-for-forensic-dna-
testing-laboratories.pdf/view/ (hereinafter, “ QAS”). 
30 QAS,  Standard  15.  
31 Fundamentals of Forensic DNA  T yping, p. 271. 
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National Accreditation Board (ANAB).32 In addition, DNA analysts must undergo semiannual 
proficiency testing.33 DNA analysts who do not pass their semiannual proficiency tests are not to 
be al owed to enter profiles into CODIS.34 Laboratories are also required to conduct two reviews 
of al  DNA profiles before they are entered into CODIS.35 
Currently, as prescribed by federal law (see “Quality Assurance and Proficiency Testing 
Standards”), only public laboratories that meet the QAS can submit DNA profiles to the NDIS. 
However, public laboratories are al owed to outsource casework to private laboratories and, 
provided certain standards are met, upload those analyses (i.e., profiles) to the NDIS. Al  private 
laboratories that conduct DNA testing for public laboratories must also be accredited, be audited 
annual y, and adhere to the requirements of the QAS.36 Public laboratories are required to conduct 
an initial  site visit to each private laboratory they contract with to conduct DNA analyses.37 If the 
public laboratory signs a contract with a private laboratory that is longer than one year, the public 
laboratory must conduct an annual site visit.38 Public laboratories are also required to review al  
outsourced DNA profiles generated by private laboratories.39 The review by the public laboratory 
is in addition to the two reviews private laboratories are required to conduct per the QAS. 
An offender profile in a DNA database consists of up to 40 numbers representing the data for the 
two al eles  at the 20 STR loci, an agency identification number, a sample identification number, 
and an identifier for the analyst that entered the information.40 Most jurisdictions retain the DNA 
sample used to generate the profile placed in CODIS.41 DNA samples are usual y retained for 
quality assurance purposes, such as confirming a hit made using the NDIS, and it al ows 
jurisdictions to retest the sample if new technology is developed in the future.42 
Privacy advocates are concerned that stored DNA samples include a wealth of genetic 
information that could be misused.43 States and the federal government have sought to prevent the 
unauthorized use of DNA samples. Some states have criminal penalties in place for individuals 
who misuse DNA samples collected for law enforcement purposes.44 Under current federal law, 
anyone who improperly discloses genetic information or misuses a DNA sample collected under 
federal authority is subject to a fine of up to $250,000, or imprisonment for up to one year.45 
                                              
32 CODIS  FAQs.  Note that t he American Society of Crime Laboratory Directors/Laboratory Accreditation Board 
(ASCLD/LAB)  and Forensic Quality Services  (FQS),  although approved as distinct accrediting agencies,  are now  both 
under ANAB. 
33 QAS,  Standard  13.  
34 Fundamentals of Forensic DNA  T yping, p. 271. 
35 U.S.  Congress, House  Committee on the Judiciary, Subcommittee on Crime, T errorism, and Homeland Security, 
Testim ony of Jeffery S. Boschwitz,  Ph.D., Hearing on “ Rape Kit Backlogs: Failing  the T est of Providing Justice to 
Sexual  Assault  Survivors”, 111th Cong., 2nd sess., May 20, 2010, H.Hrg 111-115 (Washington: GPO, 2010), p. 81. 
36 QAS,  Standard  17. 
37 QAS,  Standard  17. 
38 QAS,  Standard  17. 
39 QAS,  Standard  17. 
40 Fundamentals of Forensic DNA  T yping, p. 270. 
41 Fundamentals of Forensic DNA  T yping, p. 262. 
42 Fundamentals of Forensic DNA  T yping, p. 262.  
43 T ania Simoncelli, “Dangerous Excursions: T he Case Against Expanding Forensic DNA Databases  to  Innocent 
Persons,” Journal of Law, Medicine, and Ethics, vol. 34, no. 2 (Summer  2006), p. 392 (hereinafter, “Dangerous 
Excursions”). 
44 Dangerous Excursions, p. 392. 
45 34 U.S.C.  §40706(c). 
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There are also policies that mandate DNA expungement (i.e., the destruction of DNA samples and 
removal of profiles from DNA databases) in certain cases (see “Inclusion and Expungement of 
DNA Profiles in the NDIS”). 
The number of offender profiles included in the NDIS has increased as Congress has al owed 
states to include DNA profiles from a broader range of convicted offenders and persons arrested 
for certain crimes in the NDIS. States have also amended their DNA collection laws to reflect this 
expanded authority. Approximately 17.9 mil ion convicted offender and arrestee profiles have 
been added to the NDIS since 2000.46 In addition, 1,018,948 forensic profiles have been included 
in NDIS since 2000. Since the creation of the NDIS, hits generated by searches of it have aided in 
the investigation of more than 512,917 crimes.47 
Rapid DNA Analysis 
Rapid DNA machines are portable instruments that al ow law enforcement officials to collect and 
analyze DNA samples in approximately 90 minutes with minimal training, and outside of a 
laboratory when necessary. Rapid DNA machines use samples collected from buccal (i.e., cheek) 
swabs to develop DNA profiles. The Rapid DNA Act of 2017 (Rapid DNA Act, P.L. 115-50) 
authorized the FBI to “issue standards and procedures for the use of Rapid DNA instruments and 
resulting DNA analyses.” Rapid analysis is only recommended for generating DNA profiles of 
known persons, either to upload to CODIS to search for matches to crime scene or victim 
evidence (hits) or to use in support of law enforcement investigations of relationships between 
known persons. At present, there at two Rapid DNA Systems that are approved for accredited 
laboratory use on reference samples in an accredited laboratory.48 In 2019, the FBI began pilot 
programs with select law enforcement agencies to al ow DNA profiles collected from arrestees 
during the booking process and analyzed with Rapid DNA machines to be uploaded and searched 
on the NDIS and CODIS. However, according to the FBI, there are not presently any Rapid DNA 
Booking Systems approved for law enforcement to enter into the NDIS.49 
Rapid DNA instruments are not authorized for use in processing crime scene DNA evidence.50 
There are several reasons why it is not advisable to use Rapid DNA machines for crime scene 
evidence, primary among them that such instruments destroy, or consume, the evidence they 
analyze. As a result, a crime scene sample analyzed by a Rapid DNA instrument cannot be 
retested. Crime scene evidence also is often much more complex than DNA samples obtained via 
buccal swabs.51 Crime scene samples can be damaged or degraded and may contain mixed 
                                              
46 T he FBI reports data on the number of offender, arrestee, and forensic profiles in the NDIS  in 2000 at 
https://www.fbi.gov/file-repository/combined-dna-index-system-codis-brochure.pdf/view. T he most recent data on the 
number of offender, arrestee, and forensics profiles in the NDIS  can be found at https://www.fbi.gov/services/
laboratory/biometric-analysis/codis/ndis-statistics. T he figures on the number of profiles added  since 2000 are based  on 
the reported number of offender, arrestee, and forensic profiles in the NDIS  as of July  2020. 
47 CODIS-NDIS  Statistics. 
48 Email correspondence with Federal Bureau  of Investigation, Office of Congressional Affairs, November 18, 2020. 
49 U.S.  Department of Justice, Federal Bureau  of Investigation, Rapid DNA General Information, https://www.fbi.gov/
services/laboratory/biometric-analysis/codis/rapid-dna#:~:text=
General%20Information,cheek)%20swab%20without%20human%20intervention.&text=The%20use%20of%20the%2
0term,cheek)%20swab%E2%80%9D%20is%20intentional (hereinafter, “ Rapid DNA General Information”). 
50 National District Attorneys Association, NDAA position statement on rapid DNA, January 30, 2018, 
https://dps.alaska.gov/getmedia/fb933229-8e52-4cf8-8fe0-cb72d5e039e3/NDAA-Statement-on-Use-of-Rapid-DNA-
T echnology-2018.pdf;.aspx. 
51 Rapid DNA  General Information. 
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genetic material that cannot be distinguished by Rapid DNA machines. The use of Rapid DNA 
analysis on these more complex crime scene samples may thus lead to inconclusive or incorrect 
DNA analysis that a forensic lab may not be able to rectify because the sample is destroyed. Thus, 
the FBI has stated that crime scene samples must be “processed by an accredited forensic DNA 
laboratory that follows the FBI Quality Assurance Standards for Forensic DNA Testing 
Laboratories to be eligible  for upload and/or search in CODIS.”52 However, reporting indicates 
that some state and local law enforcement agencies have used Rapid DNA instruments to analyze 
crime scene samples and to compare profiles in SDIS and LDIS systems.53 As stated above, the 
FBI has certified specific Rapid DNA systems used only on reference samples in certified labs for 
searches in the NDIS. The ability to enter DNA profiles generated via Rapid DNA instruments 
and collected from any source, including crime scene samples, into SDISs and LDISs is 
determined by state and local law. 
DNA Backlog 
One consequence of the expansion of the collection and use of DNA in criminal investigations 
has been an increased burden on crime laboratories that can lead to a backlog of untested DNA 
samples. Delays in processing DNA evidence can impede efforts to apprehend and prosecute 
al eged offenders and exonerate wrongfully convicted individuals. Persistent backlogs can also 
result in crime laboratories prioritizing DNA analysis for violent offenses, such as homicide or 
sexual assault, over other offenses, such as property crimes, or in law enforcement agencies 
establishing policies stating that biological  evidence is not to be collected for minor offenses.54 
Backlogs are best considered in the context of each crime laboratory’s capacity, size, and 
workload.55 For example, if there are two laboratories and the first laboratory has a backlog of 
casework that is three times the size of the casework backlog in the second laboratory, the 
backlog for the first laboratory might not be as daunting if the first laboratory’s turnaround time is 
twice as fast as the second laboratory and the analysts in the first laboratory are more productive 
(i.e., each analyst analyzes more cases per month). 
Forensic Casework 
Although there is no complete nationwide account of unsubmitted DNA evidence currently in the 
possession of law enforcement, there are several resources that shed light on the backlog.56 A 
report on federal grants that address the DNA backlog determined that in states and units of local 
government that were awarded DNA Capacity Enhancement and Backlog Reduction (CEBR) 
grants between 2011 and 2017, the total requests for DNA analysis increased from about 250,000 
                                              
52 Rapid DNA  General Information. 
53 Heather Murphy, “Coming Soon to a Police Station Near You: T he DNA ‘Magic Box’”, New  York Times, January 
21, 2019, https://www.nytimes.com/2019/01/21/science/dna-crime-gene-technology.html; Maura Dolan, “ ‘Rapid 
DNA’ promises breakthroughs in solving crimes. So why  does it face a backlash?”, Los Angeles Times, September 25, 
2019, https://www.latimes.com/california/story/2019-09-24/rapid-dna-forensics-crime-police. 
54 Edwin  Zedlewski  and Mary B. Murphy, “DNA Analysis for ‘Minor’ Crimes: A Major Benefit for Law 
Enforcement,” NIJ Journal, vol. 253 (January 2006) (hereinafter, “DNA Analysis for ‘Minor’ Crimes”). 
55 Mark Nelson, Ruby  Chase, and Lindsay DePalma, Making Sense of DNA Backlog, 2012s—Myths vs. Reality, U.S. 
Department of Justice, Office of Justice Programs, National Institute of Justice, NCJ  243347, Washington, DC, 
December 2013, p. 6. 
56 U.S.  Government Accountability Office, DNA Evidence DOJ Should Improve Performance Measurement and 
Properly Design Controls  for Nationwide Grant Program , GAO-19-216, March 2019, p. 23 (hereinafter, “ DNA 
Evidence”). 
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to 300,000, or about 20%.57 Across the same period, crime labs also completed more DNA 
analysis requests, increasing from just over 200,000 completed to more than 250,000.58 However, 
the backlog for crime scene DNA analysis had increased from 91,000 requests to about 169,000, 
or 85%.59 
Another source of information on the DNA backlog is the Bureau of Justice Statistic ’s (BJS’s) 
Census of Publicly Funded Forensic Crime Laboratories (CPFCL).60 BJS compared data from the 
2014 and 2009 census collections to show how requests for DNA analysis have changed. In 2009, 
BJS reported an estimated 103,500 backlogged forensic casework analyses, which increased in 
2014 to an estimated 107,800 backlogged analyses.61 However, the estimated increase in 
backlogged forensic casework analyses was not statistical y significant. BJS data demonstrated 
that public crime laboratories completed more forensic casework samples in 2014 than in 2009. 
In 2014, public crime laboratories completed an estimated 296,000 requests for forensic casework 
analysis, up from an estimated 239,000 in 2009.62 While crime laboratories were able to process 
more cases, the requests received for forensics casework analysis increased from an estimated 
260,000 in 2009 to an estimated 333,000 in 2014.63 In both cases (i.e., completed and received 
requests), the differences between the data for 2009 and 2014 were statistical y significant.  
Convicted Offender and Arrestee Samples 
Data from BJS show that there was a statistical y significant decrease in the backlog of requests 
for analysis of convicted offender and arrestee samples. The backlog of these samples decreased 
from 502,500 in 2009 to 64,800 in 2014.64 Public crime laboratories processed an estimated 
1,027,000 convicted offender and arrestee samples in 2009 and an estimated 904,000 samples in 
2014, but the difference in processed samples is not statistical y significant.65 However, there was 
a statistical y significant decrease in requests for analysis of convicted offender and arrestee 
samples (1,053,000 requests were received in 2009 compared to 908,000 in 2014).66 This 
decrease in requests may reflect the fact that arrests national y declined by 20% between 2009 
and 2014.67 
                                              
57 DNA Evidence, p. 18.  
58 DNA Evidence, p. 18. 
59 DNA Evidence, p. 17. 
60 Matthew R. Durose et al., Publicly Funded Forensic Crime Laboratories: Resources and Services, 2014, U.S. 
Department of Justice, Office of Justice Programs, Bureau  of Justice Statistics, NCJ  250151, Washington, DC, 
November 2016 (hereinafter, “ Publicly Funded  Forensic Crime Laboratories”). 
61 Publicly Funded  Forensic Crime Laboratories, T able 6. A request  for analysis was  considered to be backlogged  if it 
was  not examined and reported to the submitting agency within 30 days.  
62 Publicly Funded  Forensic Crime Laboratories, T able 4. 
63 Publicly Funded  Forensic Crime Laboratories, T able 4. 
64 Publicly Funded  Forensic Crime Laboratories, T able 6. 
65 Publicly Funded  Forensic Crime Laboratories, T able 4. 
66 Publicly Funded  Forensic Crime Laboratories, T able 4. 
67 Department of Justice, Federal Bureau  of Investigat ion, Crime Data Explorer, https://crime-data-
explorer.fr.cloud.gov/explorer/national/united-states/arrest.  
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Sexual Assault Kits (SAKs) 
In recent years, increased attention has been paid to DNA backlogs in cases involving sexual 
assault (often referred to as the rape kit backlog).68 According to the National Institute of Justice 
(NIJ), sexual assault kits are typical y considered backlogged if they have been submitted to a 
crime laboratory but remain untested after 30 days.69 A 2018 study estimated that 200,000 sexual 
assault kits remain untested in the custody of police departments.70 The results from four studies 
funded by the NIJ indicated that 25%-50% of tested sexual assault kits generated CODIS-eligible 
DNA profiles and 50%-60% of those CODIS profiles resulted in a hit.71 
Federal Law 
Although state law dictates which profiles wil  be included in each state’s DNA database, federal 
law provides for the collection of DNA samples from certain federal offenders for analysis and 
inclusion in the NDIS. Federal law also dictates which profiles included in state databases can be 
uploaded into the NDIS. Federal law also states that agencies participating in the NDIS must meet 
certain specified standards. In addition, federal law provides for post-conviction DNA testing for 
federal offenders. The following section summarizes current federal law s pertaining to how DNA 
is used in a criminal justice capacity. 
Quality Assurance and Proficiency Testing Standards 
Under current law,72 the FBI is required to issue (and “revise from time to time”) the QAS, 
including standards for testing the proficiency of forensic laboratories and forensic analysts in 
conducting DNA analyses.73 By law, the QAS must specify the criteria for quality assurance and 
proficiency tests to be applied to the various types of DNA analyses conducted by forensic 
laboratories.74 The Rapid DNA Act (P.L. 115-50) also requires the FBI to issue standards and 
procedures for the use of Rapid DNA instruments and the resulting analyses. The QAS must 
include a system for grading proficiency testing performance to determine whether a laboratory is 
performing acceptably.75 Under current law, FBI personnel who perform DNA analyses must 
undergo semiannual external proficiency testing by a DNA proficiency testing program that meets 
the standards set in the QAS.76 
According to the FBI, the QAS also describes the minimum standards for a laboratory engaged in 
forensic DNA analysis and/or databasing.77 The QAS includes minimum laboratory standards for 
                                              
68 Gillian  M. Pinchevsky, “Criminal Justice Considerations for Unsubmitted and Untested Sexual  Assault Kits: A 
Review  of the Literature and Suggestions  for Moving Forward,” Criminal Justice Policy Review,  vol. 29, no. 9 (2018). 
69 National Institute of Justice, Sexual Assault Kits:  Using Science to Find Solutions, https://www.nij.gov/unsubmitted-
kits/.  
70 Rebecca  Campbell et al., “T ested at Last: How DNA  Evidence in Untested Rape Kits Can Identify Offenders and 
Serial  Sexual  Assaults  ,” Journal of Interpersonal Violence, vol. 33, no. 24 (2018). 
71 Gillian  M. Pinchevsky, “Criminal Justice Considerations for Unsubmitted and Untested Sexual  Assault Kits: A 
Review  of the Literature and Suggestions  for Moving Forward,” Criminal Justice  Policy Review, vol. 29, no. 9 (2018). 
72 34 U.S.C.  §12591(a)(2). 
73 T he most recent QAS took effect on July 1, 2020. 
74 34 U.S.C.  §12591(a)(3). 
75 34 U.S.C.  §12591(a)(3). 
76 34 U.S.C.  §12593(a)(1)(A). 
77 CODIS  FAQs. 
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the following areas: organization, personnel, facilities, evidence or sample control, validation, 
analytical procedures, equipment calibration and maintenance, reports, review, proficiency 
testing, corrective action, audits, safety, and outsourcing.78 
Inclusion and Expungement of DNA Profiles in the NDIS 
The Violent Crime Control and Law Enforcement Act of 1994 (P.L. 103-322) authorized the FBI 
to establish an index of DNA profiles (i.e., NDIS).  
Under current law,79 the NDIS can contain the DNA profiles of samples 
  taken from individuals convicted of or charged with a crime, or collected under 
applicable legal authorities (e.g., people arrested for crimes), except for DNA 
samples that are voluntarily submitted solely for elimination purposes (i.e., to 
exclude someone as a suspect); 
  recovered from crime scenes; 
  recovered from unidentified human remains; and 
  voluntarily contributed from relatives of missing persons.80 
The NDIS can only include DNA profiles  
  based on analyses performed by or on behalf of a criminal justice agency or the 
Department of Defense (DOD) in accordance with available standards that satisfy 
or exceed the FBI’s published QAS; 
  that are prepared by laboratories that (1) have been accredited by a nonprofit 
professional organization of persons actively involved in forensic science and 
national y recognized within the forensic science community, and (2) undergo 
external audits, not less than once every other year, that demonstrate compliance 
with the FBI’s QAS;81  
  that are prepared by accredited crime laboratories using rapid DNA instruments 
approved by the FBI in compliance with FBI published standards and procedures 
per the Rapid DNA Act82; and 
  that are maintained by federal, state, and local criminal justice agencies or the 
DOD pursuant to rules that al ow the disclosure of profiles only to other criminal 
                                              
78 CODIS  FAQs. 
79 34 U.S.C.  §12592(a). 
80 Under the Violent Crime Control and Law  Enforcement Act of 1994 ( P.L. 103-322), the NDIS was  only to include 
analyses of DNA samples collected from (1) individuals  convicted of crimes, (2) crime scenes, and (3) unidentified 
human remains. T he Justice for All  Act of 2004 (P.L. 108-405) amended the authorizing legislation for the NDIS to 
allow  analyses of DNA samples collected from persons who have been charged in an indictment or information with a 
crime and other persons whose DNA samples are collected under  applicable legal authorities to be included  in the 
NDIS,  provided that profiles from arrestees who have not been charged with a crime and samples that are voluntarily 
submitted solely for elimination purposes are not included  in the NDIS. T he Violence Against Women and Department 
of Justice  Reauthorization Act of 2005 (P.L. 109-162) amended the authorizing legislation for the NDIS to allow 
analyses of samples collected from arrestees to be included  in the NDIS.   
81 According to the FBI, American Association for Laboratory Accreditation (A2LA), and ANSI -ASQ  National 
Accreditation Board (ANAB:  T he American Society of Crime Laboratory Directors/Laboratory Accreditation Board 
(ASCLD/LAB)  and Forensic Quality Services  (FQS),  approved separately as accrediting agencies are now part of 
ANAB) are recognized as accrediting agencies. See  CODIS  FAQs. 
82 As of the cover date of this report , the FBI does not allow rapid DNA profiles in the NDIS.   
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justice agencies for identification purposes, judicial proceedings, criminal 
defense purposes, and, if personal y identifiable  information is removed, for 
research and quality control purposes.83 
Under current law, the FBI is required to expunge the profile of an individual  who had a DNA 
profile entered into the NDIS on the basis of being convicted for a qualifying federal offense (see 
below) if the individual  provides a certified copy of a final court order showing that the 
conviction was overturned.84 Also, the FBI is required to expunge the profile of an individual  who 
had a DNA profile entered into the NDIS on the basis of being arrested under the authority of the 
United States if the individual  provides a certified copy of a final court order that establishes that 
the charge was dismissed or resulted in an acquittal, or that no charge was filed within the 
applicable time period.85 Under current law, DOD is required to expunge the profile of an 
individual  who had a DNA profile entered into the NDIS on the basis of being convicted of a 
qualifying military offense (see below) if the individual provides a certified copy of a final court 
order showing that the conviction was overturned.86 In each case, it is the individual  whose DNA 
profile has been submitted to the NDIS who is responsible for seeking a DNA expungement.  
As a condition of having access to the NDIS, states must also have a procedure to expunge a 
DNA profile from the state’s database based on the same conditions applicable to a profile being 
expunged from the NDIS.87 As with the federal government, many states place the responsibility 
for pursuing DNA expungement on the people whose DNA profiles have been submitted to an 
SDIS.88 Thus, people who are eligible for expungement are required to know that expungements 
are possible (many states do not require that arrestees be notified of this possibility), correctly 
identify their own eligibility,  and both pay for and complete al  administrative steps (often 
including an expungement hearing).89 DNA expungement is relatively rare. In a sample of four 
states that place the responsibility for pursuing DNA expungement on the arrestee, less than 1% 
of eligible  samples were expunged.90 However, there are states (e.g., Connecticut, Tennessee, 
Texas, Utah, North Carolina, Maryland) that have automatic DNA expungement policies that 
require the state to initiate expungement proceedings.91 
Collection of DNA Samples from Certain Federal, District of 
Columbia, and Military Offenders 
Under current law,92 the Attorney General is permitted to collect DNA samples from “individuals 
who are arrested, facing charges, or convicted of a crime or from non-United States citizens who 
                                              
83 34 U.S.C.  §12592(b). 
84 34 U.S.C.  §12592(d)(1)(A)(i). 
85 34 U.S.C.  §12592(d)(1)(A)(ii). 
86 10 U.S.C.  §1565(e). 
87 34 U.S.C.  §12592(d)(2)(A). 
88 National Conference of State Legislatures, DNA Arrestee Laws,  http://www.ncsl.org/Documents/cj/
ArresteeDNALaws.pdf (hereinafter, “ DNA Arrestee Laws”).  
89 Elizabeth E. Joh, “T he Myth of Arrestee DNA Expungement,” University of Pennsylvania Law Review Online, vol. 
164, (2015), p. 51 (hereinafter, “The Myth of Arrestee DNA Expungement ”). 
90 T he Myth of Arrestee DNA Expungement , p. 57. 
91 DNA Arrestee Laws. 
92 34 U.S.C.  §40702(a)(1)(A). 
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are detained under the authority of the United States.”93 In addition, the Bureau of Prisons (BOP) 
is required to collect a DNA sample from each federal prisoner who is, or has been, convicted of a 
felony, a sexual abuse crime under chapter 109A of title 18 of the U.S. Code, a crime of 
violence,94 or any attempt or conspiracy to commit any of these crimes.95 Federal probation 
officers responsible for supervising individuals on probation, parole, or supervised release are 
required to collect DNA samples from individuals who are, or have been, convicted of any of the 
crimes outlined above.96 Collected samples are required to be submitted to the FBI for analysis 
and the resulting DNA profiles are included in the NDIS.97 The Rapid DNA Act al ows the FBI to 
waive the requirement that DNA samples be submitted to the FBI for analysis if the analysis is 
conducted with a rapid DNA instrument and the results are included in the NDIS. 
Current law contains similar provisions regarding the collection of DNA samples from District of 
Columbia offenders. BOP is required to collect a DNA sample from each prisoner who is, or has  
been, convicted of a qualifying District of Columbia offense.98 In addition, the Court Services and 
Offender Supervision Agency for the District of Columbia is required to collect DNA samples 
from individuals on probation, parole, or supervised release, who are, or have been, convicted of 
any qualifying District of Columbia offense.99 The government of the District of Columbia may 
determine which offenses under the District of Columbia Code are considered qualifying offenses 
to require a DNA sample.100 Collected samples must be submitted to the FBI for analysis and 
their resulting DNA profiles are included in the NDIS.101 The Rapid DNA Act also al ows the FBI 
to waive the requirement that DNA samples be submitted to the FBI for analysis if the analysis is 
conducted with a rapid DNA instrument and the results are included in the NDIS. 
                                              
93 T he DNA Analysis Backlog  Elimination Act of 2000 (P.L. 106-546) required BOP and U.S.  probation offices to 
collect DNA samples from anyone in their custody who was  convicted of qualifying  federal offenses. T he act defined a 
“qualifying federal offense” as murder, voluntary manslaughter, or other offenses relating to homicide; an offen se 
relating to sexual abuse,  sexual exploitation or other abuse of children, or transportation for illegal sexual  activity; an 
offense relating to peonage or slavery; kidnapping; an offense relating to robbery or burglary;  any offense committed in 
Indian country relating to murder, manslaughter, kidnapping, maiming, a felony sexual  abuse  offense, incest, arson, 
robbery, or burglary;  or any attempt or conspiracy to commit any of these crimes. T he Uniting and Strengthening 
America by Providing Appropriate T ools Required to Intercept and Obstruct T errorism (USA PAT RIOT ) Act of 2001 
(P.L. 107-56) expanded the definition of “ qualifying federal offense” to include crimes of terrorism, crimes of violence, 
or any attempt or conspiracy to commit either crime. T he Justice for All Act of 2004 ( P.L. 108-405) amended the 
definition of “qualifying federal offense” to include any felony, sexual abuse  offense, crime of violence, or attempt or 
conspiracy to commit any of these crimes. T he Violence Against Women and Department of Justice Reauthorization 
Act of 2005 (P.L. 109-162) authorized DOJ to collect DNA samples from arrestees and non -citizens who are detained 
under the authority of the United States. T he Adam Walsh Child  Protection and Safety Act of 2006 ( P.L. 109-248) 
authorized DOJ  to also collect DNA samples from individuals  facing charges in addition to those who have been 
arrested or convicted. 
94 As defined  at 18 U.S.C.  §16. 
95 34 U.S.C.  §40702(a)(1)(B). 
96 34 U.S.C.  §40702(a)(2). 
97 34 U.S.C.  §40702(b). 
98 34 U.S.C.  §40703(a)(1). 
99 34 U.S.C.  §40703(a)(2). 
100 34 U.S.C.  §40703(d). 
101 34 U.S.C.  §40703(b). T he following are considered qualifying  offenses under the D.C. Code:  (1) any felony; (2) any 
offense for which the penalty is greater than one year imprisonment; (3) lewd, indecent, or obscene acts knowingly 
committed in the presence of a child  under 16 years of age (D.C. Code  §22 -1312(b)); (4) certain obscene activities 
involving minors (D.C. Code §22-2201); (5) sexual performances using a minor (D.C. Code  §22 -3102); (6) 
misdemeanor sexual  abuse  (D.C. Code  §22-3006); (7) misdemeanor sexual abuse  of child or a minor (D.C. Code §22 -
3010.01); or (8) any attempt or conspiracy to commit any of these cr imes. D.C. Code  §22-4151. 
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Under current law,102 the DOD is required to collect DNA samples from each member of the 
Armed Forces who is, or has been, convicted of an offense under the Uniform Code of Military 
Justice for which a sentence of confinement of more than one year can be imposed, or of any 
other offense under the Uniform Code of Military Justice that is comparable to the offenses for 
which a DNA sample can be collected from a federal offender.103 DOD is required to conduct an 
analysis of the collected sample and submit the results to the FBI for inclusion in the NDIS.104 
Post-conviction DNA Testing 
The Justice for Al  Act of 2004 (Justice for Al  Act, P.L. 108-405) established procedures for post-
conviction DNA testing in federal courts. Under current law,105 upon a written motion from an 
individual  sentenced for a federal offense (hereinafter, “applicant”), the court must order DNA 
testing of evidence if al  of the following apply: 
  The applicant asserts, under penalty of perjury, that the applicant is actual y 
innocent of the federal crime for which the applicant was sentenced, or another 
federal or state offense, if (1) “the evidence was entered during a federal death 
sentence hearing and exoneration for the offense would entitle the applicant to a 
reduced sentence or a new sentencing hearing”; or (2) “in the case of a [s]tate 
offense, the applicant demonstrates that there is no adequate remedy under [s]tate 
law to permit DNA testing of the … evidence … and, to the extent available,  the 
applicant has exhausted al  remedies available  under [s]tate law for requesting 
DNA testing of … evidence.” 
  The specified evidence to be tested was secured in relation to the investigation or 
prosecution of the federal or state crime for which the applicant claims to be 
innocent. 
  The evidence to be tested (1) “was not previously subjected to DNA testing and 
the applicant did not knowingly fail to request DNA testing of that evidence in a 
prior motion for postconviction DNA testing”; or (2) “was previously subjected 
to DNA testing and the applicant is requesting DNA testing using a new method 
or technology that is substantial y more probative than prior testing.” 
  The evidence to be tested “is in the possession of the [g]overnment and has been 
subject to a chain of custody and retained under conditions sufficient to ensure 
that such evidence has not been substituted, contaminated, tampered with, 
replaced, or altered in any respect” that would affect the DNA testing. 
  The proposed DNA testing is “reasonable in scope, uses scientifical y sound 
methods, and is consistent with accepted forensic practices.” 
  The applicant “identifies a theory of defense that is not inconsistent with an 
affirmative defense presented at trial and would establish the actual innocence of 
the applicant.” 
                                              
102 10 U.S.C.  §1565(a)(1). 
103 T he requirement to collect DNA samples for people convicted of certain offenses under the Uniform Code of 
Military Justice is separate from the DNA samples the Department of Defense collects to aid in th e identification of 
human remains. 
104 10 U.S.C.  §1565(b). 
105 18 U.S.C.  §3600(a). 
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  If the applicant was “convicted following a trial, the identity of the perpetrator 
was at issue in the trial.” 
  The proposed DNA testing may produce new material evidence that would 
support the affirmative defense theory presented at trial and raise a reasonable 
probability that the applicant did not commit the crime. 
  The applicant certifies that he or she wil  provide a DNA sample for comparison 
purposes. 
  The motion is made in a timely fashion.106 
If the court orders DNA testing, the testing is carried out by the FBI.107 However, the court can 
order DNA testing to be conducted by another “qualified laboratory if the court makes al  
necessary orders to ensure the integrity of the … evidence and the reliability  of the testing process 
and results.”108 The cost of any DNA testing is borne by the applicant unless the applicant is 
indigent; in that case, the cost of DNA testing is borne by the government.109 
The results of any post-conviction DNA test must be simultaneously provided to the court, 
applicant, and U.S. Attorney’s office.110 If the DNA test excludes the applicant as the source of 
the biological  evidence, the DNA profile is required to be run through CODIS, assuming that the 
analysis was conducted in a manner consistent with the FBI’s QAS, to see if the probative sample 
matches any profiles in the NDIS. The results of this search are to be simultaneously provided to 
the court, applicant, and U.S. Attorney’s office. If the test results ordered by the court are 
“inconclusive or show that the applicant was the source of the tested evidence, the applicant’s 
DNA profile may be retained in the NDIS.”111 Moreover, if the test results show that the applicant 
was not the source of the tested evidence, and a comparison of the applicant’s DNA profile with 
other forensic profiles in the NDIS results in a match, the Department of Justice (DOJ) is to 
contact the appropriate agency and preserve the applicant’s DNA sample.112 However, if the test 
results exclude the applicant as the source of the tested evidence, and a comparison between the 
applicant’s DNA profile and forensic profiles in the NDIS does not result in a match, DOJ must 
destroy the applicant’s DNA sample and ensure that the applicant’s DNA profile is not stored in 
the NDIS if there is no other legal authority to retain the profile in the NDIS.113 
                                              
106 T here is a rebuttable presumption of timeliness if the motion is made within 60 months of the enactment of the 
Justice for All  Act of 2004 (October 30, 2004) or within 36 months of conviction, whichever comes later. T he 
presumption of timeliness may be rebutted upon a showing  that the applicant’s motion for DNA testing is based  solely 
upon information used in a previously denied  motion or of clear and convincing evidence that the applicant’s filing is 
done solely to cause  delay or harass. For any motion th at is not made within 60 months of the enactment of the Justice 
for All Act of 2004 or within 36 months of conviction, there is a rebuttable presumption against timeliness. T he 
presumption against timeliness can be rebutted upon the court’s finding (1) that the applicant was or is incompetent and 
such incompetence substantially contributed to the delay in the applicant’s motion for a DNA test; (2) the evidence to 
be tested is  newly discovered  DNA evidence; (3) that the applicant’s motion is not based solely  upon the applicant’s 
own assertion of innocence and, after considering all relevant facts and circumstances surrounding  the motion, a denial 
would  result in a manifest injustice; or (4) upon good cause  shown. 18 U.S.C.  §3600(a)(10)(B).  
107 18 U.S.C.  §3600(c)(1). 
108 18 U.S.C.  §3600(c)(2). 
109 18 U.S.C.  §3600(c)(3). 
110 18 U.S.C.  §3600(e)(1). 
111 18 U.S.C.  §3600(e)(3)(A). 
112 18 U.S.C.  §3600(e)(3)(B). 
113 18 U.S.C.  §3600(e)(3)(C). 
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If the results of the DNA test are inconclusive, the court can order further testing, if appropriate, 
or it can deny the applicant relief.114 If the results of the DNA test demonstrate that the applicant 
was the source of the evidence tested, the applicant is denied relief, and on a motion of the 
government, the court can determine whether the applicant’s claim of actual innocence was false. 
If the court finds the claim was false, it can 
  hold the applicant in contempt of court;  
  assess against the applicant any cost of DNA testing;  
  forward the findings to BOP, who may wholly, or in part, deny the applicant’s 
good conduct time;115 
  if the applicant is eligible  for parole, forward the finding to the U.S. Parole 
Commission so the commission can deny parole on the basis of the finding; or 
  if the test results relate to a state offense, forward the findings to the appropriate 
state official.116 
Under current law, if the applicant is convicted of making false assertions relating to post-
conviction DNA testing, the applicant is to be sentenced to no less than three years’ 
imprisonment, to run consecutively with any other term of imprisonment the applicant is 
serving.117 
If the results of the DNA testing demonstrate that the applicant was not the source of the tested 
evidence presented as a part of the case against the applicant, the applicant can file a motion for a 
new trial or resentencing, as appropriate, notwithstanding any law that would bar the motion as 
untimely.118 Under current law, 
the court shall grant the motion  of  the applicant for  a  new  trial  or  resentencing, as 
appropriate, if the DNA test results, when considered with all other evidence in the case 
(regardless of whether such evidence was introduced at trial),  establish by compelling 
evidence that a new trial would result in an acquittal of— 
(A) in the case of a motion for a new trial, the Federal offense for which the applicant is 
sentenced to imprisonment or death; and 
(B) in the case of a motion for resentencing, another Federal or State offense, if evidence 
of such offense was admitted during a Federal sentencing hearing and exoneration of such 
offense would entitle the applicant to a reduced sentence or a new sentencing proceeding.119 
                                              
114 18 U.S.C.  §3600(f)(1). 
115 Each prisoner serving a term of imprisonment of more than one year, but not prisoners serving a life sentence, can 
receive a good time credit of up to 54 days per year to count toward serving the sentence. T he amount of the credit is 
subject  to the determination of BOP. 18 U.S.C. §3624(b). 
116 18 U.S.C.  §3600(f)(2). 
117 18 U.S.C.  §3600(f)(3). 
118 18 U.S.C.  §3600(g)(1). 
119 18 U.S.C.  §3600(g)(2). 
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DNA Exonerations 
The first DNA exoneration occurred in 1989, and since then 375 persons across 37 states have been exonerated 
using DNA.120 These exonerees  were on average 26.6 years old at the time of their conviction and were 
incarcerated for an average of 14 years.  Sixty percent of the exonerees  were African American.  One hundred and 
thirty of the exonerees  were wrongful y convicted for murder,  and 21 served  time on death row.  Twenty-nine 
percent of these convictions involved a false confession,  and 31% of those who gave false confessions were  18 
years old or younger when they were  arrested and 9% had known mental health and/or mental capacity issues.   
Preservation of Biological Evidence 
Most law enforcement agencies and forensic laboratories retain the DNA samples from which 
they generated CODIS profiles to confirm the accuracy of a hit made in the NDIS and provide for 
a retest of the sample if new technology is developed.121 The Justice for Al  Act P.L. 108-405, 
among other things, established standards for preserving biological evidence. Under current 
law,122 the federal government is required to preserve biological evidence123 that was secured in 
the investigation or prosecution of a federal offense, if a defendant was imprisoned for the 
offense, unless124 
  “after a conviction becomes final and the defendant has exhausted al  
opportunities for direct review of the conviction, the defendant is notified that the 
evidence may be destroyed and the defendant does not file a motion [for post-
conviction DNA testing] within 180 days of receipt of notice”; 
  “the evidence must be returned to its rightful owner, or it is of such size, bulk, or 
physical character as to render retention impracticable and the [g]overnment 
takes reasonable measures to remove and preserve portions of the evidence 
sufficient to permit future DNA testing”; or 
  the evidence has been the subject of post-conviction DNA testing (see above) and 
the results of the testing demonstrate that the defendant was the source of the 
evidence. 
Grants for DNA-Related Programs 
Several grant programs provide assistance to state and local governments for forensic sciences. 
The bulk of the programs focus on providing state and local governments with funding to reduce 
the backlog of forensic and offender samples waiting to be processed and entered into the NDIS. 
However, some grant programs provide funding for other purposes, such as offsetting the cost of 
providing post-conviction DNA testing. This section of the report provides a brief overview of 
grants for forensic sciences.  
                                              
120 T he Innocence Project, DNA exonerations in the United States, https://www.innocenceproject.org/dna-exonerations-
in-the-united-states/. 
121 Fundamentals of Forensic DNA  T yping, p. 262. 
122 18 U.S.C.  §3600A(a). 
123 Biological evidence is defined  as a sexual  assault forensic examination kit, or semen, blood, saliva, hair, skin tissue, 
or other identified biological  material. 18 U.S.C.  §3600A(b). 
124 18 U.S.C.  §3600A(c). 
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Debbie Smith DNA Backlog Grant Program 
The Debbie Smith DNA Backlog Grant Program (Debbie Smith grants) provides grants to state 
and local governments for nine major purposes: 
1.  analyzing  DNA samples “collected under applicable  legal  authority” for 
inclusion in CODIS;125 
2.  analyzing  DNA samples from crime scenes for inclusion in CODIS, if possible 
prioritizing  “samples from rape kits, samples from other sexual assault evidence, 
and samples taken in cases without an identified suspect”126; 
3.  increasing the capacity of state and local laboratories to complete DNA analyses; 
4.  DNA sample collection;  
5.  improving the timeliness of DNA  analysis for crime scene samples, including 
those from incidents of sexual assault and violent crimes;  
6.  establishing procedures for DNA collection from arrestees as consistent with 
federal, state, local, or tribal law;  
7.  auditing  untested samples from sexual assault evidence in the possession of state 
and local governments; 
8.  improving law enforcement agencies’ practices and timeliness for DNA 
collection and processing, particularly for sexual assault and other violent crimes; 
and  
9.  improving state and local prosecutors’ capacity to “address the backlog of violent 
crime cases in which suspects have been identified through DNA evidence.”127 
The Katie Sepich Enhanced DNA Collection Act of 2012 (P.L. 112-253) amended the Debbie 
Smith program to set aside up to $10 mil ion  of the amount appropriated for Debbie Smith grants 
for FY2013-FY2015 to assist states and territories with the costs associated with collecting DNA 
samples from arrestees (assuming there is statutory authority in the state to collect DNA samples 
from people arrested for certain offenses).128 The Sexual Assault Forensic Evidence Reporting Act 
of 2013 (the SAFER Act of 2013, Title X of P.L. 113-4) added two new purposes for the Debbie 
Smith grants (listed seventh and eighth in the list above). 
The Attorney General is required to award these funds using a formula and grant conditions that 
maximize the use of DNA technology to solve crimes and to address the needs of jurisdictions 
with large backlogs.129 In making these awards, the Attorney General is directed to weigh three 
aspects of a given jurisdiction: the number of offender and forensic samples awaiting analysis, the 
population, and the number of violent crimes.130 Current law requires DOJ to award not less than 
0.5% of the total amount appropriated each fiscal year to each state and the District of 
                                              
125 34 U.S.C.  §40701(a). 
126 34 U.S.C.  §40701(a). 
127 34 U.S.C.  §40701(a). 
128 According to the National Conference of State Legislatures, as of 2018 (the most recent report), 31 states have laws 
allowing  for the collection and analysis of DNA samples from arrestees. For more detail, see http://www.ncsl.org/
Documents/cj/Arrestee_DNA_Laws.pdf.  
129 34 U.S.C.  §40701(c). 
130 34 U.S.C.  §40701(c). 
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Columbia.131 Each territory132 is to receive 0.125% of the total appropriation.133 DOJ may award 
not more than 1% of total grant funding each fiscal year to offset the cost of accrediting and 
auditing laboratories.134 
Agencies receiving a grant under the program are required to certify that DNA analyses are 
conducted in laboratories that satisfy the FBI’s QAS and are operated either by a state or local 
government, or by a private laboratory under contract with the state or local government.135 
Grants for analyses of DNA samples can be made in the form of a contract or voucher for 
laboratory services at nonprofit or for-profit laboratories that satisfy the QAS and have been 
approved by the Attorney General.136 
State and local governments receiving funding under the program are required to submit a report 
to DOJ with a summary of the activities, an assessment of whether such activities are meeting the 
needs identified in the grant application, and other information the Attorney General may require.  
The SAFER Act of 2013 established a series of conditions for states or units of local government 
receiving a grant under the Debbie Smith program to conduct an audit of their sexual assault 
evidence. The act, among other things, requires states and local governments receiving grants for 
this purpose to (1) submit a plan for performing an audit of samples, (2) provide an estimate of 
the number of samples, (3) complete the audit within one year of receiving the grant, and (4) 
submit a report to DOJ every 60 days for at least one year after the audit is completed that 
provides data on the number of samples in the state’s or unit of local government’s possession 
along with data on new sexual assault evidence the state or local government receives and how 
those samples are being processed. 
The SAFER Act of 2013 also requires the FBI, in consultation with federal, state, and local law 
enforcement agencies, to develop protocols and practices for the accurate, timely, and effective 
collection and processing of DNA evidence, including protocols and practices specific to sexual 
assault cases. The protocols and practices are required to address (1) what evidence should be 
collected by law enforcement and forwarded for testing and the order in which that evidence 
should be tested, (2) a reasonable period of time for evidence to be forwarded to a laboratory for 
testing, (3) a reasonable period of time in which each stage of laboratory testing should be 
conducted, (4) a system to encourage communication between actors in the criminal justice 
system (e.g., law enforcement, courts, laboratory personnel, and crime victims) about the status of 
evidence testing, and (5) standards for audits of sexual assault evidence in the possession of state 
and local governments. The SAFER Act of 2017 reauthorized and extended these provisions until 
FY2023 (P.L. 115-107). 
Debbie Smith grants were original y authorized under the Justice for Al  Act (P.L. 108-405). The 
law amended the DNA Backlog Elimination  Act of 2000,137 authorizing appropriations of $151 
mil ion  for each fiscal year from FY2004 to FY2009. The program was reauthorized under the 
Debbie Smith Reauthorization Act of 2008 (P.L. 110-360), which authorized appropriations of 
$151 mil ion  from FY2009 to FY2014. The Debbie Smith Reauthorization Act of 2014 (P.L. 113-
                                              
131 34 U.S.C.  §40701(c). 
132 T he territories are as follows:  U.S.  Virgin  Islands,  American Samoa, Guam,  and the Northern Mariana Islands.  
133 34 U.S.C.  §40701(c). 
134 34 U.S.C.  §40701(k). 
135 34 U.S.C.  §40701(d). 
136 34 U.S.C.  §40701(d). 
137 T he DNA Backlog  Elimination Act of 2000 (P.L. 106-546) authorized grants to increase the capacity of state and 
local government laboratories to conduct DNA analysis of bio logical samples from crime scenes.  
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182) extended the $151 mil ion  per fiscal year authorization until FY2019. The Debbie Smith 
Reauthorization Act of 2019 (P.L. 116-104) reauthorized the program at $151 mil ion per fiscal 
year until FY2024. For FY2021, Congress appropriated $110 mil ion for the Debbie Smith 
program. 
Sexual Assault Forensic Exam Program 
This program provides grants for training, technical assistance, education, equipment, and 
information relating to the identification, collection, preservation, analysis, and use of DNA 
samples and evidence by medical personnel and those treating victims of sexual assault. The 
Debbie Smith Reauthorization Act of 2019 (P.L. 116-104) reauthorized the program at $30 
mil ion  per fiscal year until FY2024. In FY2021, Congress appropriated $4 mil ion for this 
program.  
DNA Training and Education for Law Enforcement, Correctional 
Personnel, and Court Officers Program 
This program requires the Attorney General to make grants to provide training, technical 
assistance, education, and information regarding the identification, collection, preservation, 
analysis, and use of DNA samples and evidence by law enforcement personnel, court officers, 
forensic science professionals, and corrections personnel. The Debbie Smith Reauthorization Act 
of 2019 (P.L. 116-104) reauthorized the program at more than $12 mil ion per fiscal year until 
FY2024. In FY2021, Congress al owed up to 4% of funds appropriated for the Debbie Smith 
program to be used for this purpose.  
Kirk Bloodsworth Post-Conviction DNA Testing Grant Program 
The Kirk Bloodsworth DNA Post-Conviction DNA Testing Grant program was authorized by the 
Justice for Al  Act (P.L. 108-405). The act authorized the Attorney General to make grants to 
states to help defray the costs of post-conviction DNA testing programs that may establish 
innocence for violent felony offenses. The act authorized appropriations of $5 mil ion each fiscal 
year from FY2005 to FY2009. The Justice for Al  Reauthorization Act of 2016 (P.L. 114-324) 
reauthorized appropriations for this program at $5 mil ion  per fiscal year for FY2017 to FY2021. 
For FY2021, Congress appropriated $8 mil ion for this program.  
DNA Research and Development Grants 
The Justice for Al  Act authorized grants for research and development for improving forensic 
DNA technology, including increasing the accuracy and efficiency of DNA analysis, decreasing 
the time and expense of conducting DNA analysis, and increasing its portability. In addition, the 
law authorized grants for demonstration projects to evaluate the use of DNA technology in 
conjunction with other forensic analyses. The act authorized funding of $15 mil ion each fiscal 
year for FY2005 to FY2009. The Justice for Al  Reauthorization Act of 2016 (P.L. 114-324) 
reauthorized appropriations for this program at $5 mil ion  per year for FY2017 to FY2021. 
Congress did not appropriate funds for this program in FY2021.  
Sexual Assault Kit Initiative (SAKI) 
SAKI funds address both the current backlog of sexual assault kits (SAKs) and efforts to prevent 
any future backlogs. SAKI funds may be used to inventory and test unsubmitted SAKs, pursue 
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new investigations and prosecutions, support victims, create evidence-tracking systems, train law 
enforcement in sexual assault investigations, fund research on the outcomes of sexual assault 
cases, and “increase collection of offender DNA for CODIS upload purposes (in full adherence to 
the laws in the jurisdiction), that may lead to the identification of serious and serial sex 
offenders.”138 Since the initiative’s launch in 2015, more than 130,000 SAKs have been 
inventoried, more than 71,000 kits have been submitted to labs for testing, and about 11,000 DNA 
hits have been made in CODIS.139 For FY2021, Congress appropriated $48 mil ion for this 
initiative.   
Emmett Till Cold Case Investigations Program 
Under this program, DOJ is authorized to make grants to state or local law enforcement agencies 
for expenses associated with the investigation and prosecution of criminal offenses involving civil 
rights violations that occurred not later than December 31, 1979,140 and resulted in a death. The 
Emmett Til  Unsolved Civil  Rights Crime Act of 2007 (P.L. 110-344) authorized $2.0 mil ion 
each fiscal year from FY2008 to FY2017 for this program. The Emmett Til  Unsolved Civil 
Rights Crimes Reauthorization Act of 2016 (P.L. 114-325) reauthorized this program at $2 
mil ion  each fiscal year from FY2017 to FY2027. For FY2021, Congress appropriated $2 mil ion 
for this program. 
Table 1. Appropriations for DNA-Related Programs, FY2016-FY2021 
(in mil ions of dol ars) 
 
FY2016 
FY2017 
FY2018 
FY2019 
FY2020 
FY2021 
Debbie Smith DNA Backlog Grant 
117.0a 
117.0a 
120.0a 
120.0a 
102.0a 
110.0a 
Program 
Sexual Assault Forensic  Exam Program 
4.0 
4.0 
4.0 
4.0 
4.0 
4.0 
Kirk  Bloodsworth Post-Conviction 
4.0 
4.0 
6.0 
6.0 
7.0 
8.0 
DNA Testing Grant Program 
DNA Research and Development 
— 
— 
— 
— 
— 
— 
Grants  
Sexual Assault Kit Initiative 
45.0 
45.0 
47.5 
48.0 
48.0 
48.0 
Emmett Til  Cold Case Investigations 
— 
— 
— 
— 
2.0 
2.0 
Program 
Source: FY2016 enacted taken from P.L.  114-113. FY2017 enacted taken from P.L.  115-31. FY2018 enacted 
taken from P.L.  115-141. FY2019 enacted taken from P.L.  116-6. FY2020 enacted taken from P.L.  116-93. FY2021 
enacted taken from P.L. 116-260.  
a.  DOJ is authorized to use up to 4% of the funding provided for the Debbie Smith DNA Backlog  Grant 
program for the purposes authorized under the DNA Training and Education for Law Enforcement, 
Correctional  Personnel,  and Court Officers  Program   
 
 
                                              
138 Department of Justice, Bureau of Justice Programs, Sexual  Assault  Kit Initiative (SAKI), Overview, 
https://bja.ojp.gov/program/sexual-assault -kit-initiative-saki/overview (hereinafter, “SAKI Overview”). 
139 SAKI  Overview. 
140 T his date was  originally set for December 31, 1969. T he 2016 reauthorization changed the year to 1979.  
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Going Forward 
As highlighted above, the use of DNA in the criminal justice system is constantly evolving to take 
advantage of the rapid pace of scientific and technological improvements. There are several 
issues raised here that policymakers may wish to consider. See the following, for example:  
  Rapid DNA Tests. Rapid DNA technology offers an opportunity to process 
certain types of DNA samples much more quickly and potential y lessen the 
demands on crime labs. Congress might consider legislation or grant funding on 
the use of this technology. However, Rapid DNA also has important limitations, 
particularly in its usefulness for crime scene samples. Thus, Congress might also 
consider legislation aligning with FBI guidance prohibiting the use of Rapid 
DNA machines on crime scene DNA samples. Final y, Immigration and Customs 
Enforcement (ICE) recently expanded a program to use Rapid DNA tests to 
verify familial  relationships between people “entering or attempting to enter” the 
United States at the southwest border.141 Congress might question whether DNA 
profiles obtained by ICE should be stored in CODIS, particularly profiles created 
for minors. 
  DNA Expungement. Congress might consider the low rate of DNA 
expungement discussed earlier in this report by authorizing procedures for 
automatic, or state-initiated, expungement of DNA obtained from arrestees who 
are never charged, are acquitted, or whose conviction was overturned. 
Developing a process by which individuals can have their DNA expunged from 
an SDIS is currently a condition for access to the NDIS. The Katie Sepich 
Enhanced DNA Collection Act of 2012 (P.L. 112-253) included conditions that 
grantees must provide written notification of DNA expungement policies to 
people who had samples collected, list eligibility  requirements and instructions 
for requesting expungement on a public website, and make expungement 
decisions within 90 days of receiving an expungement request. Congress might 
consider attaching similar provisions to future legislation or federal grants; 
however, adopting more stringent expungement policies may be costly for states 
in terms of both staff and resources required.  
  Sexual Assault Kit Backlog. Congress has created several federal grants to 
address the sexual assault kit backlog; however, policymakers could examine 
further actions such as developing an Electronic Evidence Exchange Standard 
(see the NIJ’s handbook of National Best Practices for Sexual Assault Kits for 
additional considerations).142 
 
                                              
141 U.S.  Immigrations and Customs Enforcement, “Ice Awards New  Contract for Rapid DNA T esting at Southwest  
Border, Expands Pilot Program,” press release, June, 18, 2019, https://www.ice.gov/news/releases/ice-awards-new-
contract -rapid-dna-testing-southwest -border-expands-pilot -program. 
142 National Institute of Justice, “National Best Practices for Sexual Assault Kits: A Multidisciplinary Approach,” 
https://www.ncjrs.gov/pdffiles1/nij/250384.pdf. 
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Author Information 
 
Emily  J. Hanson 
   
Analyst in Social Policy 
    
 
 
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Congressional Research Service  
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